Yes, online casinos often impose minimum and maximum limits on the amount you can deposit and withdraw in a single transaction. Deposits are generally tied to a minimum of $10 or $20.
Withdrawals are more changeable, with some casinos limiting the amount you can withdraw in a day, week, and month. For example, you could be limited to $1,000 in a single transaction or $5,000 over a 30 day period.